富百科-九游会ag

safered® nucleic acid gel stain is the third generation nonmutagenic fluorescent nucleic acid gel stain to replace the highly toxic traditional ethidium bromide (etbr) for staining dsdna, ssdna or rna in agarose gels or polyacrylamide gels. the third part’s ames test by wuxi pharmacy shows that safered and dugreen are nonmutagenic for gel staining. dugreen and safered nucleic acid gel stain are highly sensitive than etbr either as precast gel stains or post gel stains. we can directly replace eb with safered® without changing the existing imaging system since safered® and eb have the similar spectra. safered® can also be used to stain dsdna, ssdna or rna in polyacrylamide gel via post gel staining. gel staining with safered® is compatible with downstream applications such as gel extraction and cloning. safered® is efficiently removed from dna by phenol/chloroform extraction and ethanol precipitation.

lipo is a newly developed and proprietary reagent for the transfection of nucleic acids into eukaryotic cells. lipo has the following advantages: the highest transfection efficiency in many cell types and formats. dna-lipo complexes can be directly added to cells in culture medium (with or without serum). it is not necessary to remove dna-lipo complexes or change medium following transfection. the complexes can be removed after 4-6 hours by replacing with refresh medium (optional)

cell counting kit-8 (cck-8) utilizes the highly water-soluble tetrazolium salt to produce a water-soluble formazan dye upon reduction in the presence of an electron carrier. cell counting kit-8 is a one-bottle solution; no premixing of components is required. cell counting kit-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. cck is reduced by dehydrogenases in cells to give a yellow colored product (formazan), which is soluble in the tissue culture medium. the amount of the formazan dye generated by the activity of dehydrogenases in cells is directly proportional to the number of living cells. the detection sensitivity of cck-8 is higher than other tetrazolium salts such as mtt, xtt, mts or wst-1.

the west femto ecl substrate is an extremely sensitive enhanced chemiluminescent substrate for detecting horseradish peroxidase (hrp) on immunoblots. this substrate’s extremely intense signal output enables detection of low femtogram amounts of antigen. furthermore, this substrate requires substantial dilutions of the primary and secondary antibodies. the sensitivity, intensity and duration of the signal allow for easy detection of hrp using photographic or other imaging methods. blots also can be repeatedly exposed to film to obtain optimal results or stripped of the immunodetection reagents and reprobed.

dyes for small animal in vivo imaging and their labeling services

succinimidyl esters are proven to be the best reagents for amine modifications because the amide bonds that are formed are essentially identical to, and as stable as the natural peptide bonds. these reagents are generally stable and show good reactivity and selectivity with aliphatic amines.

fluorescent reagents and assay kits for monitoring cellular functions

real-time pcr is carried out in a thermal cycler with the capacity to illuminate each sample with a beam of light of at least one specified wavelength and detect the fluorescence emitted by the excited fluorophore. the pcr dyes are available such as sybr green，evagreen，rox reference.

fluorescent-labeled oligonucleotide probes. taqman probes consist of a fluorophore covalently attached to the 5’-end of the oligonucleotide probe and a quencher at the 3’-end. several different fluorophores (e.g. 6-carboxyfluorescein, acronym: fam, or tetrachlorofluorescein, acronym: tet) and quenchers (e.g. tetramethylrhodamine, acronym: tamra) are available. the quencher molecule quenches the fluorescence emitted by the fluorophore when excited by the cycler’s light source via förster resonance energy transfer (fret)

the qubit picogreen reagent is a proprietary, asymmetrical cyanine dye. free dye does not fluoresce, but upon binding to dsdna it exhibits a >1000-fold fluorescence enhancement. picogreen is 10000x more sensitive than uv absorbance methods, and highly selective for dsdna over ssdna and rna.

高分子荧光探针能大幅度提高荧光强度，抗淬灭性以及耐刷光性能。从而给生物大分子的检测过程和生物成像带来极大的便利。

clinical detection reagents for sysmex hematology analyzer，routine blood five classification：wbc white blood cell coun；hct ；ly% lymphocyte；rbc red blood cell .hgb hemoglobin 医学 ivd 体外诊断产品-五分类血球试剂(临床批号：苏常械备20153084号)