cy7
structure
mw = 720.94
properties | |
excitation maximum (nm) | 747 |
emission maximum (nm) | 774 |
extinction coefficient (m-1 cm-1) | 200 000 * |
* measured in phosphate-buffered saline, mujumdar, r. b.，etc. bioconjugate chem. 1993_4_105-111.
carbocyanine dyes amine-reactive
cyanine dyes are a series of reactive fluorescent dyes that are widely used for labeling peptides, proteins, nucleotides and nucleic acids. cyanine dyes are a single family of bright fluorescent signals with good water-solubility. unlike fluoresceins and hydroxycoumarins, their excitations and emissions are ph-independent. among the cyanine dyes, cyanine 3 dyes have fluorescence enhanced upon conjugating to proteins while other cyanine have fluorescence reduced due to the quenching effect of electron-rich amino acids (such as trp, his and tyr etc) in proteins. in general, the cyanine dyes have the following features:
good water solubility enables labeling reactions conveniently run in aquesous media without using organic solvents (such as dmf or dmso) as co-solvent
low non-specific binding of resultant conjugates facilitate the assay development
a wide range of excitation and emission wavelengths makes them available for versatile fluorescence instruments
cat# | name | size | ex (nm) | em (nm) | soluble in |
1050 | cyanine 3 acid | 5 mg | 548 nm | 562 nm | water |
1051 | cyanine 3 acid, mono-nhs ester | 1 mg | 548 nm | 562 nm | water |
1053 | cyanine 5 acid | 5 mg | 646 nm | 664 nm | water |
1054 | cyanine 5 acid, mono-nhs ester | 1 mg | 646 nm | 664 nm | water |
1056 | cyanine 5.5 acid | 5 mg | 673 nm | 692 nm | water |
1057 | cyanine 5.5 acid, mono-nhs ester | 1 mg | 673 nm | 692 nm | water |
1058 | cyanine 7 acid | 5 mg | 747 nm | 774 nm | water |
1059 | cyanine 7 acid, mono-nhs ester | 1 mg | 747 nm | 774 nm | water |
| name | cy7 | ||
|---|---|---|---|
| cat# | 1058-5mg | cas# | n/a |
| storage# | −20°c干燥避光 | shelf life# | 12个月 |
| ex(nm)# | 747 | em(nm)# | 774 |
| mw# | 720.94 | solvent# | n/a |
| name | cy7 |
|---|---|
| cat# | 1058-5mg |
| cas# | n/a |
| storage# | −20°c干燥避光 |
| shelf life# | 12个月 |
| ex(nm)# | 747 |
| em(nm)# | 774 |
| mw# | 720.94 |
| solvent# | n/a |