chemical phosphorylation reagent i (cpr i)
cat# | size | price | mw | abs | em | soluble in | storage |
6001 | 1 g | $395 | 656.77 | n/a | n/a | mecn | f/d/l |
features and biological applicationsthis reagent can be used to create the 3'-phosphate by adding it as the first addition to the support in labeling oligonucleotides with fluorescent dyes. after ammonia deprotection the oligo will have the 3'-phosphate attached to the 2nd base added during synthesis. both the support base and the cpr are cleaved. the dmt-group is removed during the ammonium hydroxide deprotection and thus is not available for poly-pak purification. |
1. xu y, lee sa, kutateladze tg, sbrissa d, shisheva a, prestwich gd. (2006) chemical synthesis and molecular recognition of phosphatase-resistant analogues of phosphatidylinositol-3-phosphate. j am chem soc, 128, 885.
2. ohkubo a, ezawa y, seio k, sekine m. (2004) o-selectivity and utility of phosphorylation mediated by phosphite triester intermediates in the n-unprotected phosphoramidite method. j am chem soc, 126, 10884.
3. tsuruoka h, shohda k, wada t, sekine m. (2000) synthesis and conformational properties of oligonucleotides incorporating 2'-o-phosphorylated ribonucleotides as structural motifs of pre-trna splicing intermediates. j org chem, 65, 7479.
4. olejnik j, krzymanska-olejnik e, rothschild kj. (1996) photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides. nucleic acids res, 24, 361.
5. mora n, lacombe jm, pavia aa. (1995) a new approach to phosphoserine, phosphothreonine and phosphotyrosine synthons and to thiophospho analogs. stepwise synthesis of mono- and multiphosphorylated phosphopeptides related to src-protein kinase. int j pept protein res, 45, 53.
6. boumendjel a, miller sp. (1994) synthesis of sphingosine-1-phosphate and dihydrosphingosine-1-phosphate. j lipid res, 35, 2305.
7. kitas e, kung e, bannwarth w. (1994) chemical synthesis of o-thiophosphotyrosyl peptides. int j pept protein res, 43, 146.
8. tegge w, ballou ce. (1992) syntheses of d-myo-inositol 1,4,5-trisphosphate affinity ligands. carbohydr res, 230, 63.
9. perich jw, reynolds ec. (1991) fmoc/solid-phase synthesis of tyr(p)-containing peptides through t-butyl phosphate protection. int j pept protein res, 37, 572.
10. lacombe jm, andriamanampisoa f, pavia aa. (1990) solid-phase synthesis of peptides containing phosphoserine using phosphate tert.-butyl protecting group. int j pept protein res, 36, 275.
| name | chemical phosphorylation reagent i (cpr i) | ||
|---|---|---|---|
| cat# | 6001-100mg | cas# | n/a |
| storage# | −20°c desiccated and minimize light exposure | shelf life# | 12 months |
| ex(nm)# | n/a | em(nm)# | n/a |
| mw# | 656.77 | solvent# | mecn，dmso |
| name | chemical phosphorylation reagent i (cpr i) |
|---|---|
| cat# | 6001-100mg |
| cas# | n/a |
| storage# | −20°c desiccated and minimize light exposure |
| shelf life# | 12 months |
| ex(nm)# | n/a |
| em(nm)# | n/a |
| mw# | 656.77 |
| solvent# | mecn，dmso |