chemical phosphorylation reagent ii (cpr ii)
cat# | size | price | mw | abs | em | soluble in | storage |
6005 | 1 g | $395 | 722.8 | n/a | n/a | mecn | f/d/l |
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features and biological applicationsthis chemical phosphorylation reagent (cpr ii) contains a dmt group that can be left on the oligonucleotide and used for rapid purification of oligonucleotide 5'-phosphates by the popular dmtr-on technique, which employs disposable rp cartridges or “trityl-on” rp hplc purification. the dmtr group is removed with aqueous acid (e.g., 2%tfa in the case of cartridge purification) and the remaining linker is then eliminated after brief treatment with aqueous ammonium hydroxide (12 -15% ammonium hydroxide at room temperature for 15 minutes) to yield the 5'-phosphate. |
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| name | chemical phosphorylation reagent ii (cpr ii) | ||
|---|---|---|---|
| cat# | 6005-100mg | cas# | n/a |
| storage# | −20°c desiccated and minimize light exposure | shelf life# | 12 months |
| ex(nm)# | n/a | em(nm)# | n/a |
| mw# | 722.8 | solvent# | mecn， dmso |
| name | chemical phosphorylation reagent ii (cpr ii) |
|---|---|
| cat# | 6005-100mg |
| cas# | n/a |
| storage# | −20°c desiccated and minimize light exposure |
| shelf life# | 12 months |
| ex(nm)# | n/a |
| em(nm)# | n/a |
| mw# | 722.8 |
| solvent# | mecn， dmso |