6-九游会ag

    the light-absorbing properties of tamra, and spectral overlap with several commonly used fluorophores - including fam, hex, tet and joe, make it useful as a fret acceptor for the dual labeled fret probes such as molecular beacons. tamra has been used extensively for real-time pcr and other molecular diagnostic applications. oligonucleotides can be labeled with tamra using two distinct methodologies. under standard deprotection conditions, tamra is not sufficiently stable; the molecule degrades in the presence of ammonium hydroxide. if standard deprotection is required, the oligonucleotide is normally synthesized with an amino group at either the 3'-, or 5'-end and post-labeled with tamra succinimidyl ester. oligonucleotides synthesized using ultramild monomers can also be labeled directly with tamra, either internally by substituting any suitable dt residue with tamra-dt-ce phosphoramidite, or at the 3'-end using 3'-tamra cpg support. subsequent deprotection of the oligo using potassium carbonate in methanol adequately removes the base protecting groups, leaving the tamra intact. alternatively the deprotection of 1:1:2 t-butylamine/methanol/water allows the use of regular monomers.

product number:                    6051

lot number:                           dcm018003

product name:                        6-tamra cpg *1000 å*

chemical structure:

appearance:                           purple particles 

degree of dye content:           36 μmole/g 

shipping condition:               room temperature

storage conditions:                -20 °c; desiccation is recommended.

expiration date:                      12 months upon receiving

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