7-aad [7-aminoactinomycin d]
cat# | size | price | mw | abs | em | soluble in | storage |
17501 | 1 mg | $75 | 1270.43 | 546 nm | 647 nm | dmso | f/d/l |
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features and biological applications7-amino actinomycin d (7-aad) is a non-permeant dye that can be used to identify non-viable cells. cells with damaged plasma membranes or with impaired/no cell metabolism are unable to prevent the dye from entering the cell. once inside the cell, the dyes bind to intracellular dna producing highly fluorescent adducts which identify the cells as non-viable. it is widely used in flow cytometry. 7-aad is excited by the 488 nm laser line of an argon laser with fluorescence detected above 650 nm. although the emission intensity of 7-aad is lower than that of pi, the longer wavelength emission may make it more useful for multiplexing assays in combination with other 488 nm-excited fluorochromes such as fitc and pe. |
1. kovalev ae, iakovenko aa, vekshin nl. (2004) [a study of interaction of 7-aminoactinomycin d with dna by fluorescence correlation spectroscopy]. biofizika, 49, 1030.
2. gaforio jj, serrano mj, algarra i, ortega e, alvarez de cienfuegos g. (2002) phagocytosis of apoptotic cells assessed by flow cytometry using 7-aminoactinomycin d. cytometry, 49, 8.
3. lecoeur h, de oliveira-pinto lm, gougeon ml. (2002) multiparametric flow cytometric analysis of biochemical and functional events associated with apoptosis and oncosis using the 7-aminoactinomycin d assay. j immunol methods, 265, 81.
4. linden e, skoglund p, rundquist i. (1997) accessibility of 7-aminoactinomycin d to lymphocyte nuclei after paraformaldehyde fixation. cytometry, 27, 92.
5. loborg h, linden e, lonn a, skoglund p, rundquist i. (1995) high affinity binding of 7-aminoactinomycin d and 4',6-diamidino-2-phenylindole to human neutrophilic granulocytes and lymphocytes. cytometry, 20, 296.
6. rundquist i. (1993) equilibrium binding of dapi and 7-aminoactinomycin d to chromatin of cultured cells. cytometry, 14, 610.
7. stokke t, holte h, smeland eb, lie so, steen hb. (1992) differential chromatin structure-dependent binding of 7-aminoactinomycin d in normal and malignant bone marrow hematopoietic cells. cancer res, 52, 5007.
8. wadkins rm, jovin tm. (1991) actinomycin d and 7-aminoactinomycin d binding to single-stranded dna. biochemistry, 30, 9469.
| name | 7-aad [7-aminoactinomycin d] | ||
|---|---|---|---|
| cat# | 17501-1mg | cas# | n/a |
| storage# | f/d/l | shelf life# | 12 months |
| ex(nm)# | 546 | em(nm)# | 647 |
| mw# | 1270.43 | solvent# | dmso |
| name | 7-aad [7-aminoactinomycin d] |
|---|---|
| cat# | 17501-1mg |
| cas# | n/a |
| storage# | f/d/l |
| shelf life# | 12 months |
| ex(nm)# | 546 |
| em(nm)# | 647 |
| mw# | 1270.43 |
| solvent# | dmso |